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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

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Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Figure 2. Immunocytochemistry staining of PCK2 using Anti-PCK2 Antibody (A04772-2). ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPCK2
  • SizePrice
Figure 1. Western blotting validation for Anti-Cofilin1/2 (T88) CFL2 Antibody A04773T88 Western blot (WB) analysis of Cofilin1/2 (T88) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:Mouse heart tissue lysate Lane3:Rat heart tissue lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetCFL1
  • SizePrice
Figure 1. Western blotting validation for Anti-PRRX1 Antibody A04774-1 Western blot (WB) analysis of PRRX1 polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lyate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPRRX1
  • SizePrice
Western blot analysis of PRRX1 in HeLa cell lysate with PRRX1 antibody at 1 microg/ml.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityChicken, Human, Mouse, Rat
TargetPRRX1
  • SizePrice
Figure 1. Western blotting validation for Anti-CC2D1A Antibody A04775 Western blot analysis of extracts of various cells
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCC2D1A
  • SizePrice
Figure 1. Western blot analysis of HADHB using anti-HADHB antibody (A04776-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHB antigen affinity purified polyclonal antibody (Catalog # A04776-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADHB at approximately 45 kDa. The expected band size for HADHB is at 51 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHADHB
  • SizePrice
Figure 1. Western blot analysis of HADHB using anti-HADHB antibody (A04776-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHB antigen affinity purified polyclonal antibody (Catalog # A04776-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADHB at approximately 45 kDa. The expected band size for HADHB is at 51 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHADHB
  • SizePrice

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