Pepsin (ready to use)

GeneTex

Antigen retrieval condition-Pepsin (ready to use)

9

Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS. Remove buffer, add 2-4 drops of Pepsin reagent to cover tissue section. Incubate at 37oC or at 56oC for 5-10 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5-10 minutes are sufficient at the above temperatures. In some cases it may require 15 minute.). Discard Pepsin reagent, wash 3-5 times with buffer.

Research Use Only

Liquid

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

2°C to 8°C

12352204