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ANTXR1 / TEM8 Antibody

LS-C39569
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetANTXR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ANTXR1 / TEM8 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human TEM8. Using a colorimetric detection system, the detection limit for rhTEM8 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human TEM8. The detection limit for rhTEM8 is approximately 5 ng/well.. ELISA, WB Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human TEM8. Using a colorimetric detection system, the detection limit for rhTEM8 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human TEM8. The detection limit for rhTEM8 is approximately 5 ng/well.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84168
  • Target name
    ANTXR1
  • Target description
    ANTXR cell adhesion molecule 1
  • Target synonyms
    2310008J16Rik; 2810405N18Rik; anthrax toxin receptor 1; ATR; GAPO; TEM8; tumor endothelial marker 8
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TEM8/ATR/ANTXR1 using anti-TEM8/ATR/ANTXR1 antibody (A01563-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEM8/ATR/ANTXR1 antigen affinity purified polyclonal antibody (Catalog # A01563-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEM8/ATR/ANTXR1 at approximately 80 kDa. The expected band size for TEM8/ATR/ANTXR1 is at 63 kDa.
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