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Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEM8 Polyclonal Antibody, Unconjugated (bs-15583R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEM8 Polyclonal Antibody, Unconjugated (bs-15583R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEM8 Polyclonal Antibody, Unconjugated (bs-15583R) at 1:200 overnight at 4°C, DAB staining.

TEM8 Polyclonal Antibody

BS-15583R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetANTXR1
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Overview

  • Supplier
    Bioss
  • Product Name
    TEM8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID84168
  • Target name
    ANTXR1
  • Target description
    ANTXR cell adhesion molecule 1
  • Target synonyms
    ATR, GAPO, TEM8, anthrax toxin receptor 1, 2310008J16Rik, 2810405N18Rik, tumor endothelial marker 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6X2
  • Protein Name
    Anthrax toxin receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Taguchi K, Onoe T, Yoshida T, et al. Isolation of tumor endothelial cells from murine cancer. J Immunol Methods. 2019,464:105-113. doi: 10.1016/j.jim.2018.11.005
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TEM8/ATR/ANTXR1 using anti-TEM8/ATR/ANTXR1 antibody (A01563-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEM8/ATR/ANTXR1 antigen affinity purified polyclonal antibody (Catalog # A01563-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEM8/ATR/ANTXR1 at approximately 80 kDa. The expected band size for TEM8/ATR/ANTXR1 is at 63 kDa.
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