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Western Blot analysis of NIH-3T3 cells using Annexin X Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Annexin X Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Annexin X Polyclonal Antibody

ANXA10 Antibody

CSB-PA000882
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetANXA10
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANXA10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11199
  • Target name
    ANXA10
  • Target description
    annexin A10
  • Target synonyms
    annexin 14; annexin A10; annexin-10; ANX14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJ72
  • Protein Name
    Annexin A10
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A10/ANXA10 antigen affinity purified polyclonal antibody (Catalog # A13560-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A10/ANXA10 at approximately 36 kDa. The expected band size for Annexin A10/ANXA10 is at 37 kDa.
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References
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Lane 1: A549 cell lysates; Lane 2: Hela cell lysates; Lane 3: U87MG cell lysates probed with Annexin A10 Polyclonal Antibody, Unconjugated (bs-5127R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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