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Western Blot analysis of HepG2 cells using Annexin III Polyclonal Antibody
Western Blot analysis of HepG2 cells using Annexin III Polyclonal Antibody
Western Blot analysis of HepG2 cells using Annexin III Polyclonal Antibody

ANXA3 Antibody

CSB-PA000880
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetANXA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANXA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID306
  • Target name
    ANXA3
  • Target description
    annexin A3
  • Target synonyms
    35-alpha calcimedin; annexin A3; Annexin III (lipocortin III); annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha); annexin-3; ANX3; calcimedin 35-alpha; inositol 1,2-cyclic phosphate 2-phosphohydrolase; lipocortin III; PAP-III; placental anticoagulant protein III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12429
  • Protein Name
    Annexin A3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANXA3 using anti-ANXA3 antibody (A04796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # A04796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA3 at approximately 36 kDa. The expected band size for ANXA3 is at 36 kDa.
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