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ProductsBack/ANXA6 Antibody (OAAF01731)

ANXA6 Antibody (OAAF01731)

OAAF01731
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANXA6
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ANXA6 Antibody (OAAF01731)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID309
  • Target name
    ANXA6
  • Target description
    annexin A6
  • Target synonyms
    67 kDa calelectrin; annexin A6; annexin VI (p68); annexin-6; ANX6; calcium-binding protein p68; calelectrin; calphobindin II; CBP68; chromobindin-20; CPB-II; lipocortin VI; p68; p70; testis secretory sperm-binding protein Li 198a
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.
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