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Western Blot analysis of 293 cells using Annexin VI Polyclonal Antibody
Western Blot analysis of 293 cells using Annexin VI Polyclonal Antibody
Western Blot analysis of 293 cells using Annexin VI Polyclonal Antibody

ANXA6 Antibody

CSB-PA000881
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANXA6
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANXA6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID309
  • Target name
    ANXA6
  • Target description
    annexin A6
  • Target synonyms
    ANX6, CBP68, CPB-II, p68, p70, annexin A6, 67 kDa calelectrin, annexin VI (p68), annexin-6, calcium-binding protein p68, calelectrin, calphobindin II, chromobindin-20, lipocortin VI, testis secretory sperm-binding protein Li 198a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08133
  • Protein Name
    Annexin A6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.
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