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AP-1 / JUND Antibody (phospho-Ser255)

LS-C416577
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetJUND
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AP-1 / JUND Antibody (phospho-Ser255)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3727
  • Target name
    JUND
  • Target description
    JunD proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, transcription factor JunD, activator protein 1, jun D proto-oncogene, transcription factor AP-1 subunit JunD, transcription factor jun-D
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JunD using anti-JunD antibody (A05609-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U2OS whole cell lysates, Lane 8: human U251 whole cell lysates, Lane 9: rat lung tissue lysates, Lane 10: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JunD antigen affinity purified polyclonal antibody (Catalog # A05609-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JunD at approximately 38 kDa, 42 kDa. The expected band size for JunD is at 35 kDa.
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