AP chromogen BCIP/NBT (ready-to-use)
GTX27468
Product group Assays
Overview
- SupplierGeneTex
- Product NameAP chromogen BCIP/NBT (ready-to-use)
- Delivery Days Customer9
- Application Supplier NoteSee ProtocolBCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.
- ApplicationsDot Blot, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- FormulationLiquid
- Scientific DescriptionAlkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.
- Storage Instruction2°C to 8°C,RT
- UNSPSC41116133
References
- CXCR3 promotes the production of IgG1 autoantibodies but is not essential for the development of lupus nephritis in NZB/NZW mice. Moser K et al., 2012 Apr, Arthritis RheumRead more