Bio-Connect

AP chromogen BCIP/NBT (ready-to-use)

Research Use Only
GTX27468
GeneTex
Product group Assays
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Overview

  • Supplier
    GeneTex
  • Product Name
    AP chromogen BCIP/NBT (ready-to-use)
  • Delivery Days Customer
    9
  • Application Supplier Note
    See ProtocolBCIP/NBT system is based on hydrolysis of BCIP and reduction of NBT producing a deep purple reaction product. Reaction is several times more sensitive than existing products with little or no background staining. The reaction product is extremely stable and does not fade when exposed to light.
  • Applications
    Dot Blot, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Formulation
    Liquid
  • Scientific Description
    Alkaline Phosphatase based immunostaining systems are gaining wide acceptance among pathologists because of their relatively high signal to noise ratio. 5-bromo,4-chloro,3-indolylphosphate (BCIP)/nitroblue tetrazolium (NBT) substrate for alkaline phosphatase-based detection systems, is a commonly used substrate chromogen. BCIP acts as the substrate for alkaline phosphatase, and the NBT enhances the purplish-brown final color of the precipitates at the reaction sites.
  • Storage Instruction
    2°C to 8°C,RT
  • UNSPSC
    41116133

References

  • CXCR3 promotes the production of IgG1 autoantibodies but is not essential for the development of lupus nephritis in NZB/NZW mice. Moser K et al., 2012 Apr, Arthritis Rheum
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