Western blot using GeneTex Affinity Purified anti-AP1G1 antibody (GTX23706) shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 microg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 microg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.
Western blot using GeneTex Affinity Purified anti-AP1G1 antibody (GTX23706) shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 microg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 microg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.
AP1G1 antibody
GTX23706
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAP1G1
Overview
- SupplierGeneTex
- Product NameAP1G1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IP: 1:100. ELISA: 1:2000-1:8000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID164
- Target nameAP1G1
- Target descriptionadaptor related protein complex 1 subunit gamma 1
- Target synonymsadapter-related protein complex 1 subunit gamma-1; adaptor protein complex AP-1 subunit gamma-1; adaptor related protein complex 1 gamma 1 subunit; ADTG; AP-1 complex subunit gamma-1; CLAPG1; clathrin assembly protein complex 1 gamma large chain; clathrin assembly protein complex 1 gamma-1 large chain; clathrin-associated/assembly/adaptor protein, large, gamma 1; gamma adaptin; gamma1-adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; golgi adaptor HA1/AP1 adaptin subunit gamma-1; testicular tissue protein Li 21
- HostGoat
- IsotypeIgG
- Protein IDO43747
- Protein NameAP-1 complex subunit gamma-1
- Scientific DescriptionAP1G1 is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203