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AP2A2 Antibody

LS-C748332
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAP2A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AP2A2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 73kDa/103kDa/104kDa, while the observed MW by Western blot was 104kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 73kDa/103kDa/104kDa, while the observed MW by Western blot was 104kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID161
  • Target name
    AP2A2
  • Target description
    adaptor related protein complex 2 subunit alpha 2
  • Target synonyms
    ADTAB, CLAPA2, HIP-9, HIP9, HYPJ, AP-2 complex subunit alpha-2, 100 kDa coated vesicle protein C, adapter-related protein complex 2 subunit alpha-2, adaptin, alpha B, adaptor related protein complex 2 alpha 2 subunit, alpha-adaptin C; Huntingtin interacting protein J, alpha2-adaptin, clathrin assembly protein complex 2 alpha-C large chain, clathrin-associated/assembly/adaptor protein, large, alpha 2, huntingtin yeast partner J, huntingtin-interacting protein 9, plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP2A2 using anti-AP2A2 antibody (A08527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2A2 antigen affinity purified polyclonal antibody (Catalog # A08527-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2A2 at approximately 104 kDa. The expected band size for AP2A2 is at 104 kDa.
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