Western blot using GeneTexs Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 microg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.
Western blot using GeneTexs Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 microg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.
Ap3D1 antibody
GTX23705
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAP3D1
Overview
- SupplierGeneTex
- Product NameAp3D1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. IP: 1:100. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.6 mg/ml
- ConjugateUnconjugated
- Gene ID8943
- Target nameAP3D1
- Target descriptionadaptor related protein complex 3 subunit delta 1
- Target synonymsadapter-related protein complex 3 subunit delta-1; adaptor related protein complex 3 delta 1 subunit; ADTD; AP-3 complex delta subunit, partial CDS; AP-3 complex subunit delta-1; delta adaptin; hBLVR; HPS10; subunit of putative vesicle coat adaptor complex AP-3
- HostGoat
- IsotypeIgG
- Protein IDO14617
- Protein NameAP-3 complex subunit delta-1
- Scientific DescriptionAP3D1 is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in intracellular biogenesis and trafficking of pigment granules and possibly platelet dense granules and neurotransmitter vesicles.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203