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APC Anti-Human CD83 Antibody

RD20098F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD83
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    APC Anti-Human CD83 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HB15e
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID9308
  • Target name
    CD83
  • Target description
    CD83 molecule
  • Target synonyms
    B-cell activation protein; BL11; CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); cell surface protein HB15; cell-surface glycoprotein; HB15; hCD83
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    CD83 is a 43 kD single chain type I glycoprotein also known as HB15. A member of the immunoglobulin superfamily, CD83 is expressed on a subset of dendritic cells, Langerhans cells, and weakly on activated lymphocytes. Although CD83 is thought to play a role in antigen presentation and/or lymphocyte activation, the precise function of this protein is unknown. CD83 is considered to be a useful marker for mature dendritic cells.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM22 using anti-ADAM22 antibody (A01777-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM22 antigen affinity purified polyclonal antibody (Catalog # A01777-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM22 at approximately 45 kDa. The expected band size for ADAM22 is at 23 kDa.
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