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Western Blot analysis of HepG2 cells using CD83 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CD83 Polyclonal Antibody
Western Blot analysis of HepG2 cells using CD83 Polyclonal Antibody

CD83 Antibody

CSB-PA001478
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCD83
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD83 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9308
  • Target name
    CD83
  • Target description
    CD83 molecule
  • Target synonyms
    B-cell activation protein; BL11; CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); cell surface protein HB15; cell-surface glycoprotein; HB15; hCD83
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01151
  • Protein Name
    CD83 antigen
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM22 using anti-ADAM22 antibody (A01777-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM22 antigen affinity purified polyclonal antibody (Catalog # A01777-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM22 at approximately 45 kDa. The expected band size for ADAM22 is at 23 kDa.
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A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD83 Antibody（bs-4826R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
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