Western blotting. Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa
Western blotting. Affinity Purified Rabbit anti-APC10 (GTX23629) was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa
Apc10 antibody
GTX23629
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANAPC10
Overview
- SupplierGeneTex
- Product NameApc10 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1500. IP: 1:100. ELISA: 1:15000-1:80000. IHC: 1:250-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.5 mg/ml
- ConjugateUnconjugated
- Gene ID10393
- Target nameANAPC10
- Target descriptionanaphase promoting complex subunit 10
- Target synonymsanaphase-promoting complex subunit 10; APC10; cyclosome subunit 10; DOC1
- HostRabbit
- IsotypeIgG
- Protein IDQ9UM13
- Protein NameAnaphase-promoting complex subunit 10
- Scientific DescriptionAPC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203