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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by secondary antibody, DAB, and counter-staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by secondary antibody, DAB, and counter-staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4°C, followed by secondary antibody, DAB, and counter-staining.

Apelin Polyclonal Antibody

BS-2425R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPLN
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Overview

  • Supplier
    Bioss
  • Product Name
    Apelin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8862
  • Target name
    APLN
  • Target description
    apelin
  • Target synonyms
    APEL, XNPEP2, apelin, AGTRL1 ligand, APJ endogenous ligand
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULZ1
  • Protein Name
    Apelin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Kasacka I, Piotrowska Ż, Filipek A, et al. Comparative evaluation of cannabinoid receptors, apelin and S100A6 protein in the heart of women of different age groups. BMC Cardiovasc Disord. 2018,18(1):190. doi: 10.1186/s12872-018-0923-0
    Read this paper

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Figure 1. IHC analysis of Apelin/APLN using anti-Apelin/APLN antibody (A02075-1). Apelin/APLN was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-Apelin/APLN Antibody (A02075-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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