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Western Blot analysis of 3T3 cells using APLP-2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using APLP-2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using APLP-2 Polyclonal Antibody

APLP2 Antibody

CSB-PA000903
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPLP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    APLP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID334
  • Target name
    APLP2
  • Target description
    amyloid beta precursor like protein 2
  • Target synonyms
    APLP-2, APPH, APPL2, CDEBP, amyloid beta precursor like protein 2, CDEI box-binding protein, amyloid beta (A4) precursor-like protein 2, amyloid precursor protein homolog HSD-2, sperm membrane protein YWK-II, testicular tissue protein Li 23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06481
  • Protein Name
    Amyloid-like protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of APLP2 using anti-APLP2 antibody (A01232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (Catalog # A01232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.
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