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Apoa1 Antibody, HRP conjugated

CSB-PA001913LB01RA
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityRat
TargetApoa1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Apoa1 Antibody, HRP conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID25081
  • Target name
    Apoa1
  • Target description
    apolipoprotein A1
  • Target synonyms
    apoA-I, apolipoprotein A-I, apo-AI, apolipoprotein A-1, preproapolipoprotein A-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04639
  • Protein Name
    Apolipoprotein A-I
  • Scientific Description
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Rat Heart tissue, Rat Liver tissue, Rat Spleen tissue, Rat Lung tissue, Rat Kidney tissue, Rat Brain tissue, Rat Stomach tissue All lanes: Apoa1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa
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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9845). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9845) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 24 kDa. The expected band size for APOA1 is at 30 kDa.
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