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Western Blot analysis of HeLa cells using AI-BP Polyclonal Antibody
Western Blot analysis of HeLa cells using AI-BP Polyclonal Antibody
Western Blot analysis of HeLa cells using AI-BP Polyclonal Antibody

APOA1BP Antibody

CSB-PA000834
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNAXE
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Overview

  • Supplier
    Cusabio
  • Product Name
    APOA1BP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID128240
  • Target name
    NAXE
  • Target description
    NAD(P)HX epimerase
  • Target synonyms
    AIBP, APOA1BP, PEBEL, YJEFN1, NAD(P)H-hydrate epimerase, AI-BP, apoA-I binding protein, apolipoprotein A-I-binding protein, yjeF N-terminal domain-containing protein 1, yjeF_N1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NCW5
  • Protein Name
    NAD(P)H-hydrate epimerase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NAXE using anti-NAXE antibody (A10537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse liver tissue lysaets. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAXE antigen affinity purified polyclonal antibody (Catalog # A10537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAXE at approximately 26 kDa. The expected band size for NAXE is at 32 kDa.
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