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Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using APOBEC3G Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using APOBEC3G Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using APOBEC3G Polyclonal Antibody at dilution 1:50

APOBEC3G Polyclonal Antibody

E-AB-10817
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    APOBEC3G Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
  • UNSPSC
    12352203

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Human HeLa lysates probed with Rabbit Anti-APOBEC3G Polyclonal Antibody, Unconjugated (bs-15407R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Figure 1. Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3G antigen affinity purified polyclonal antibody (Catalog # PB9985) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 46 kDa. The expected band size for APOBEC3G is at 46 kDa.
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