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ApoER2/LRP8 Rabbit mAb

A9747
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetLRP8
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Overview

  • Supplier
    ABclonal
  • Product Name
    ApoER2/LRP8 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7804
  • Target name
    LRP8
  • Target description
    LDL receptor related protein 8
  • Target synonyms
    APOER2, HSZ75190, LRP-8, MCI1, low-density lipoprotein receptor-related protein 8, ApoE receptor 2, low density lipoprotein receptor-related protein 8, apolipoprotein e receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14114
  • Protein Name
    Low-density lipoprotein receptor-related protein 8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat thymus tissue lysates, Lane 10: rat spleen tissue lysates, Lane 11: rat heart tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse thymus tissue lysates, Lane 14: mouse heart tissue lysates, Lane 15: mouse Raw264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2/LRP8 antigen affinity purified polyclonal antibody (Catalog # A03444-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ApoER2/LRP8 at approximately 106KD. The expected band size for ApoER2/LRP8 is at 106KD.
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Anti-ApoER2/LRP8 Antibody Picoband(r)A03444-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLRP8
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TargetLRP8
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Lane 1: Mouse Lung lysates; Lane 2: Rat Lung lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human Hela cell lysates probed with ApoER2 Polyclonal Antibody, Unconjugated (bs-6651R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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TargetLRP8
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ApoER2/LRP8 AntibodyCAB10517
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