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Apolipoprotein A-V Antibody

LS-C812495
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAPOA5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Apolipoprotein A-V Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID116519
  • Target name
    APOA5
  • Target description
    apolipoprotein A5
  • Target synonyms
    APOAV; apo-AV; apolipoprotein A-V; apolipoprotein A-V precursor variant 3; RAP3; regeneration-associated protein 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apolipoprotein A V/APOA5 using anti-Apolipoprotein A V/APOA5 antibody (A01242-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein A V/APOA5 antigen affinity purified polyclonal antibody (Catalog # A01242-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein A V/APOA5 at approximately 45KD. The expected band size for Apolipoprotein A V/APOA5 is at 45KD.
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