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Apolipoprotein A1 antibody

GTX44402
GeneTex
ApplicationsRadioImmunoAssay
Product group Antibodies
ReactivityMouse
TargetApoa1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Apolipoprotein A1 antibody
  • Delivery Days Customer
    9
  • Applications
    RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11806
  • Target name
    Apoa1
  • Target description
    apolipoprotein A-I
  • Target synonyms
    Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, apo-AI, apoA-I, apolipoprotein A-I, apolipoprotein A1
  • Host
    Goat
  • Isotype
    IgG
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9844). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9844) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 24 kDa. The expected band size for APOA1 is at 31 kDa.
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