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Apolipoprotein A5 antibody [4H8H8E2 (c)]

GTX83300
GeneTex
ApplicationsELISA
Product group Antibodies
TargetAPOA5
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Overview

  • Supplier
    GeneTex
  • Product Name
    Apolipoprotein A5 antibody [4H8H8E2 (c)]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4H8H8E2 (c)
  • Conjugate
    Unconjugated
  • Gene ID116519
  • Target name
    APOA5
  • Target description
    apolipoprotein A5
  • Target synonyms
    APOAV; apo-AV; apolipoprotein A-V; apolipoprotein A-V precursor variant 3; RAP3; regeneration-associated protein 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ6Q788
  • Protein Name
    Apolipoprotein A-V
  • Scientific Description
    The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apolipoprotein A V/APOA5 using anti-Apolipoprotein A V/APOA5 antibody (A01242-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein A V/APOA5 antigen affinity purified polyclonal antibody (Catalog # A01242-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein A V/APOA5 at approximately 45KD. The expected band size for Apolipoprotein A V/APOA5 is at 45KD.
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