ICC/IF analysis of HepG2 cells using GTX15663 Apolipoprotein B antibody [F2C13]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of HepG2 cells using GTX15663 Apolipoprotein B antibody [F2C13]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
Apolipoprotein B antibody [F2C13]
GTX15663
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, RadioImmunoAssay
Product group Antibodies
ReactivityHuman
TargetAPOB
Overview
- SupplierGeneTex
- Product NameApolipoprotein B antibody [F2C13]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDF2C13
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID338
- Target nameAPOB
- Target descriptionapolipoprotein B
- Target synonymsapoB-100; apoB-48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; apolipoprotein B48; FCHL2; FLDB; LDLCQ4
- HostMouse
- IsotypeIgG1
- Protein IDP04114
- Protein NameApolipoprotein B-100
- Scientific DescriptionThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203