Apolipoprotein CII

Athens Research

Apolipoprotein CII

9

In Vitro Diagnostics, Cardiovascular Research

Research Use Only

≥95% by SDS-PAGE

Apolipoprotein C-II

Apolipoprotein CII (ApoCII) is a 79-amino acid protein encoded by the APOC2 gene, serving as an essential cofactor for lipoprotein lipase (LPL) activation. This protein circulates in plasma as a component of triglyceride-rich lipoproteins (TRLs), including very low-density lipoproteins (VLDL) and chylomicrons, with normal concentrations ranging from 3–8 mg per 100 mL. ApoCII binds to LPL via its C-terminal alfa-helix (residues 58–79), stabilizing the enzyme’s catalytic domain and facilitating the hydrolysis of VLDL triglycerides into free fatty acids and glycerol, a process critical for generating intermediate-density lipoproteins (IDL). This lipolytic activity enables cellular energy uptake and regulates plasma lipid homeostasis. Deficiency in ApoCII, caused by biallelic APOC2 mutations, results in familial hyperlipoproteinemia type IB, characterized by severe hypertriglyceridemia (more then1,000 mg/dL), recurrent pancreatitis, and eruptive xanthomas. The absence of functional ApoCII impairs LPL-mediated triglyceride clearance, leading to chylomicronemia and atherosclerosis in preclinical models. Heterozygotes typically remain asymptomatic due to ApoCII’s excess physiological presence. Clinically, ApoCII deficiency is diagnosed through genetic testing and plasma apolipoprotein profiling, with acute pancreatitis managed via fresh frozen plasma infusions to replenish ApoCII. Emerging therapeutic strategies include synthetic ApoCII mimetic peptides, such as C-II-a, which combine lipid-binding and LPL-activating domains to restore triglyceride metabolism. While gene therapies like alipogene tiparvovec showed promise in preclinical studies, their clinical adoption remains limited. Additionally, ApoCII’s role in modulating lipoprotein particle interactions positions it as a biomarker for cardiovascular risk assessment and a target for lipid-lowering interventions.

more then 1 year

Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

-20C

41116100