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ProductsBack/Apolipoprotein L 1 (APOL1) Mouse Monoclonal Antibody [Clone ID: 1D4]
Western blot analysis using APOL1 antibody Cat.-No AM06309SU-N against human plasma (Lane 1).
Western blot analysis using APOL1 antibody Cat.-No AM06309SU-N against human plasma (Lane 1).
Western blot analysis using APOL1 antibody Cat.-No AM06309SU-N against human plasma (Lane 1).

Apolipoprotein L 1 (APOL1) Mouse Monoclonal Antibody [Clone ID: 1D4]

AM06309SU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAPOL1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Apolipoprotein L 1 (APOL1) Mouse Monoclonal Antibody [Clone ID: 1D4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D4
  • Gene ID8542
  • Target name
    APOL1
  • Target description
    apolipoprotein L1
  • Target synonyms
    APO-L, APOL, APOL-I, FSGS4, apolipoprotein L1, apolipoprotein L 1, apolipoprotein L1-B3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO14791
  • Protein Name
    Apolipoprotein L1
  • Scientific Description
    Apolipoprotein L 1 (APOL1) mouse monoclonal antibody, clone 1D4, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of APOL1 using anti-APOL1 antibody (A01841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human plasma tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOL1 antigen affinity purified polyclonal antibody (Catalog # A01841-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOL1 at approximately 37KD. The expected band size for APOL1 is at 44KD.
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