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WB analysis of various sample lysates using GTX30061 APRT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30061 APRT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30061 APRT antibody. Dilution : 1:1000 Loading : 25microg per lane

APRT antibody

GTX30061
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetAPRT
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Overview

  • Supplier
    GeneTex
  • Product Name
    APRT antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID353
  • Target name
    APRT
  • Target description
    adenine phosphoribosyltransferase
  • Target synonyms
    adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRTD; transphosphoribosidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07741
  • Protein Name
    Adenine phosphoribosyltransferase
  • Scientific Description
    Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IF analysis of APRT using anti-APRT antibody (A02721). APRT was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 microg/mL rabbit anti-APRT Antibody (A02721) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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