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Aqp1 Polyclonal Antibody

CAC07222
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetAQP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    AQP1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID358
  • Target name
    AQP1
  • Target description
    aquaporin 1 (Colton blood group)
  • Target synonyms
    AQP-CHIP; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group); aquaporin 1, Colton blood group antigen; aquaporin-1; aquaporin-CHIP; channel-like integral membrane protein, 28-kDa; CHIP28; CO; Colton blood group antigen; urine water channel; water channel protein for red blood cells and kidney proximal tubule
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29972
  • Protein Name
    Aquaporin-1
  • Scientific Description
    The Aqp1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF, IP.
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 1/AQP1 using anti-Aquaporin 1/AQP1 antibody (A00865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1/AQP1 antigen affinity purified polyclonal antibody (Catalog # A00865) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1/AQP1 at approximately 23 kDa. The expected band size for Aquaporin 1/AQP1 is at 23 kDa.
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