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Western Blot analysis of COS7 cells using AQP2 Polyclonal Antibody
Western Blot analysis of COS7 cells using AQP2 Polyclonal Antibody
Western Blot analysis of COS7 cells using AQP2 Polyclonal Antibody

AQP2 Antibody

CSB-PA000913
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetAQP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AQP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID359
  • Target name
    AQP2
  • Target description
    aquaporin 2
  • Target synonyms
    ADH water channel; AQP-2; AQP-CD; aquaporin 2 (collecting duct); aquaporin-2; aquaporin-CD; collecting duct water channel protein; NDI2; water channel protein for renal collecting duct; water-channel aquaporin 2; WCH-CD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41181
  • Protein Name
    Aquaporin-2
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat NRK whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse HBZY whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 2 antigen affinity purified polyclonal antibody (Catalog # PB9474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 2 at approximately 26 kDa. The expected band size for Aquaporin 2 is at 26 kDa.
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