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Western blot All lanes: Aquaporin-4 antibody at 2ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse heart tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 35 kDa
Western blot All lanes: Aquaporin-4 antibody at 2ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse heart tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 35 kDa
Western blot All lanes: Aquaporin-4 antibody at 2ug/ml Lane 1: Mouse brain tissue Lane 2: Mouse heart tissue Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 35 kDa

AQP4 Antibody

CSB-PA001964ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetAQP4
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Overview

  • Supplier
    Cusabio
  • Product Name
    AQP4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID361
  • Target name
    AQP4
  • Target description
    aquaporin 4
  • Target synonyms
    MIWC, MLC4, WCH4, hAQP4, aquaporin-4, aquaporin type4, mercurial-insensitive water channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55087
  • Protein Name
    Aquaporin-4
  • Scientific Description
    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (Catalog # A01049) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4/AQP4 at approximately 32 kDa. The expected band size for Aquaporin 4/AQP4 is at 32 kDa.
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