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Western Blot analysis of HuvEc cells using AQP5 Polyclonal Antibody
Western Blot analysis of HuvEc cells using AQP5 Polyclonal Antibody
Western Blot analysis of HuvEc cells using AQP5 Polyclonal Antibody

AQP5 Antibody

CSB-PA000915
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAQP5
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Overview

  • Supplier
    Cusabio
  • Product Name
    AQP5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID362
  • Target name
    AQP5
  • Target description
    aquaporin 5
  • Target synonyms
    AQP-5; aquaporin-5; PPKB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55064
  • Protein Name
    Aquaporin-5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 5 antigen affinity purified polyclonal antibody (Catalog # A03085) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 5 at approximately 28KD. The expected band size for Aquaporin 5 is at 28KD.
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