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IHC-P analysis of rat brain tissue using GTX37784 Aquaporin 4 antibody. Dilution : 2.5microg/ml
IHC-P analysis of rat brain tissue using GTX37784 Aquaporin 4 antibody. Dilution : 2.5microg/ml
IHC-P analysis of rat brain tissue using GTX37784 Aquaporin 4 antibody. Dilution : 2.5microg/ml

Aquaporin 4 antibody

GTX37784
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAQP4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aquaporin 4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100-2000 (based on 0.5 mg/ml). IHC-P: 1:100-1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID361
  • Target name
    AQP4
  • Target description
    aquaporin 4
  • Target synonyms
    aquaporin type4; aquaporin-4; mercurial-insensitive water channel; MIWC; WCH4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55087
  • Protein Name
    Aquaporin-4
  • Scientific Description
    This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (Catalog # A01049) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4/AQP4 at approximately 32 kDa. The expected band size for Aquaporin 4/AQP4 is at 32 kDa.
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