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Aquaporin 4 Antibody (YA3204, Rabbit)

HY-P83459
MedChem Express
TargetAQP4
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Aquaporin 4 Antibody (YA3204, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID361
  • Target name
    AQP4
  • Target description
    aquaporin 4
  • Target synonyms
    MIWC, MLC4, WCH4, hAQP4, aquaporin-4, aquaporin type4, mercurial-insensitive water channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Aquaporin 4 Antibody (YA3204) is a non-conjugated IgG antibody, targeting Aquaporin 4. Aquaporin 4 Antibody (YA3204) can be used for IHC-P experiment in human, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (Catalog # A01049) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4/AQP4 at approximately 32 kDa. The expected band size for Aquaporin 4/AQP4 is at 32 kDa.
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