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Western blot All lanes: ARF6 antibody at 2ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
Western blot All lanes: ARF6 antibody at 2ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
Western blot All lanes: ARF6 antibody at 2ug/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa

ARF6 Antibody

CSB-PA001996ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARF6
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARF6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID382
  • Target name
    ARF6
  • Target description
    ARF GTPase 6
  • Target synonyms
    ADP-ribosylation factor 6, ADP ribosylation factor 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62330
  • Protein Name
    ADP-ribosylation factor 6
  • Scientific Description
    GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ARF6 using anti-ARF6 antibody (A00927-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARF6 antigen affinity purified polyclonal antibody (Catalog # A00927-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARF6 at approximately 20KD. The expected band size for ARF6 is at 20KD.
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