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ProductsBack/ARFGAP1 Antibody (clone OTI 2C10, Carrier-free)

ARFGAP1 Antibody (clone OTI 2C10, Carrier-free)

LS-C785820
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetARFGAP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ARFGAP1 Antibody (clone OTI 2C10, Carrier-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI 2C10
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55738
  • Target name
    ARFGAP1
  • Target description
    ARF GTPase activating protein 1
  • Target synonyms
    ARF1GAP, HRIHFB2281, ADP-ribosylation factor GTPase-activating protein 1, ADP ribosylation factor GTPase activating protein 1, ARF1-directed GTPase-activating protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARFGAP1 using anti-ARFGAP1 antibody (A04959). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARFGAP1 antigen affinity purified polyclonal antibody (Catalog # A04959) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARFGAP1 at approximately 45 kDa. The expected band size for ARFGAP1 is at 45 kDa.
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