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Flow cytometric analysis of Jurkat cells with Argonaute 2 (7C3) Monoclonal Antibody (bsm-52820R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Flow cytometric analysis of Jurkat cells with Argonaute 2 (7C3) Monoclonal Antibody (bsm-52820R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Flow cytometric analysis of Jurkat cells with Argonaute 2 (7C3) Monoclonal Antibody (bsm-52820R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)

AGO2 Recombinant Antibody

BSM-52820R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAGO2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Argonaute 2 (7C3) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200), IHC()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID27161
  • Target name
    AGO2
  • Target description
    argonaute RISC catalytic component 2
  • Target synonyms
    argonaute 2, RISC catalytic component; cancer susceptibility candidate 7 (non-protein coding); CASC7; EIF2C2; eukaryotic translation initiation factor 2C, 2; LESKRES; LINC00980; long intergenic non-protein coding RNA 980; PAZ Piwi domain protein; PPD; protein argonaute-2; protein slicer; Q10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKV8
  • Protein Name
    Protein argonaute-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ago2/eIF2C2 using anti-Ago2/eIF2C2 antibody (PB9978). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ago2/eIF2C2 antigen affinity purified polyclonal antibody (Catalog # PB9978) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ago2/eIF2C2 at approximately 97 kDa. The expected band size for Ago2/eIF2C2 is at 97 kDa.
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