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Immunohistochemistry of paraffin-embedded Human lung cancer tissue using ARHGEF6 Polyclonal Antibody at dilution 1:70
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using ARHGEF6 Polyclonal Antibody at dilution 1:70
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using ARHGEF6 Polyclonal Antibody at dilution 1:70

ARHGEF6 Polyclonal Antibody

E-AB-16170
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ARHGEF6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.7mg/ml
  • Scientific Description
    Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGEF6 using anti-ARHGEF6 antibody (A05317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF6 antigen affinity purified polyclonal antibody (Catalog # A05317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF6 at approximately 87 kDa. The expected band size for ARHGEF6 is at 87 kDa.
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Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & cytosol.
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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA159616(ARHGEF6 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
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