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ARL13B Antibody

CSB-PA002074GA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARL13B
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARL13B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200894
  • Target name
    ARL13B
  • Target description
    ARF like GTPase 13B
  • Target synonyms
    ARL2L1, JBTS8, ADP-ribosylation factor-like protein 13B, ADP ribosylation factor like GTPase 13B, ADP-ribosylation factor-like 2-like 1, ARL2-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3SXY8
  • Protein Name
    ADP-ribosylation factor-like protein 13B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARL13B using anti-ARL13B antibody (A04279-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL13B antigen affinity purified polyclonal antibody (Catalog # A04279-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL13B at approximately 55 kDa. The expected band size for ARL13B is at 49 kDa.
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