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Western blot All lanes: ARPIN antibody at 0.5microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 25, 44 kDa Observed band size: 25 kDa
Western blot All lanes: ARPIN antibody at 0.5microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 25, 44 kDa Observed band size: 25 kDa
Western blot All lanes: ARPIN antibody at 0.5microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 25, 44 kDa Observed band size: 25 kDa

ARPIN Antibody

CSB-PA773801LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetARPIN
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Overview

  • Supplier
    Cusabio
  • Product Name
    ARPIN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID348110
  • Target name
    ARPIN
  • Target description
    actin related protein 2/3 complex inhibitor
  • Target synonyms
    C15orf38, arpin, UPF0552 protein C15orf38, arp2/3 inhibition protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z6K5
  • Protein Name
    Arpin
  • Scientific Description
    Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors. Participates in an incoherent feedforward loop at the lamellipodium tip where it inhibits the ARP2/2 complex in response to Rac signaling and where Rac also stimulates actin polymerization through the WAVE complex. Involved in steering cell migration by controlling its directional persistence.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARPIN using anti-ARPIN antibody (A08342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPIN antigen affinity purified polyclonal antibody (Catalog # A08342-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARPIN at approximately 35 kDa. The expected band size for ARPIN is at 25 kDa.
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