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Anti-ARSA Antibody (monoclonal, 4C10)

ORB443142
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetARSA
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Overview

  • Supplier
    Biorbyt
  • Product Name
    ARSA Antibody (monoclonal, 4C10)
  • Delivery Days Customer
    16
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml, Human Flow Cytometry (Fixed), 1-3microg/1x106 cells, Human, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C10
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID410
  • Target name
    ARSA
  • Target description
    arylsulfatase A
  • Target synonyms
    ASA, MLD, arylsulfatase A, cerebroside-sulfatase, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP15289
  • Protein Name
    Arylsulfatase A
  • Scientific Description
    Anti-ARSA Antibody (monoclonal, 4C10)
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARSA using anti-ARSA antibody (PB9477). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Pancreas Tissue Lysate, Lane 3: Rat Skeletal Muscle Tissue Lysate, Lane 4: Mouse Kidney Tissue Lysate, Lane 5: MCF-7 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSA antigen affinity purified polyclonal antibody (Catalog # PB9477) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARSA at approximately 54KD. The expected band size for ARSA is at 54KD.
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