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Aryl Hydrocarbon Receptor (AhR) Polyclonal Antibody

CAU26091
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Biomatik
  • Product Name
    Aryl Hydrocarbon Receptor (AhR) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Protein IDP41738
  • Protein Name
    Aryl hydrocarbon receptor
  • Scientific Description
    The Aryl Hydrocarbon Receptor (AhR) Polyclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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ICC/IF analysis of HeLa cells using GTX22100 AHR antibody. Green : primary antibody Red : Actin Blue : DAPI Dilution : 1:100 Fixation : 10% formalin Permibilization : 0.5% Triton-X100
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Figure 1. Western blot analysis of AHR using anti-AHR antibody (PB9419). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # PB9419) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 96 kDa. The expected band size for AHR is at 96 kDa.
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TargetAhr
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