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Immunohistochemistry of paraffin-embedded Human colon cancer tissue using ASH2L Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using ASH2L Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using ASH2L Polyclonal Antibody at dilution 1:50

ASH2L Polyclonal Antibody

E-AB-14753
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetASH2L
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Overview

  • Supplier
    Elabscience
  • Product Name
    ASH2L Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9070
  • Target name
    ASH2L
  • Target description
    ASH2 like, histone lysine methyltransferase complex subunit
  • Target synonyms
    ASH2, ASH2L1, ASH2L2, Bre2, set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ash2 (absent, small, or homeotic)-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBL3
  • Protein Name
    Set1/Ash2 histone methyltransferase complex subunit ASH2
  • Scientific Description
    The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Alternative splicing results in a shorter isoform 2, designated ASH2L2, which is missing the first 94 amino acid residues found in ASH2L1. Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. ASH2L is highly expressed in fetal liver, testis and leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASH2L using anti-ASH2L antibody (A06129-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASH2L antigen affinity purified polyclonal antibody (Catalog # A06129-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASH2L at approximately 69,80 kDa. The expected band size for ASH2L is at 69 kDa.
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