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Western Blot analysis of HepG2 cells using ASIC3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ASIC3 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ASIC3 Polyclonal Antibody

ASIC3 Antibody

CSB-PA005120
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetASIC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    ASIC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9311
  • Target name
    ASIC3
  • Target description
    acid sensing ion channel subunit 3
  • Target synonyms
    ACCN3, DRASIC, SLNAC1, TNaC1, acid-sensing ion channel 3, acid sensing (proton gated) ion channel 3, amiloride-sensitive cation channel 3, testis, modulatory subunit of ASIC2a, neuronal amiloride-sensitive cation channel 3, proton-gated cation channel subunit, testis sodium channel 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHC3
  • Protein Name
    Acid-sensing ion channel 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ASIC3 using anti-ASIC3 antibody (PA1938). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Cell Lysate, Lane 4: NEURO Cell Lysate, Lane 5: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody (Catalog # PA1938) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.
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