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Western Blot analysis of Lovo and 231 cell using ASNS Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Lovo and 231 cell using ASNS Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Lovo and 231 cell using ASNS Polyclonal Antibody at dilution of 1:1000

ASNS Polyclonal Antibody

E-AB-14755
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ASNS Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Asparagine synthetase/ASNS antigen affinity purified polyclonal antibody (Catalog # A03302-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Asparagine synthetase/ASNS at approximately 64 kDa. The expected band size for Asparagine synthetase/ASNS is at 64 kDa.
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