Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Elabscience

Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

12

0.38-72.30 IU/L

0.38 IU/L

100 min

Research Use Only

AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

2°C to 8°C

41116100