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ASUN / C12orf11 Antibody

LS-C680671
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetINTS13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ASUN / C12orf11 Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55726
  • Target name
    INTS13
  • Target description
    integrator complex subunit 13
  • Target synonyms
    ASUN, C12orf11, GCT1, Mat89Bb, NET48, SPATA30, integrator complex subunit 13, asunder, spermatogenesis regulator homolog (Drosphila), cell cycle regulator Mat89Bb homolog, germ cell tumor 1, protein asunder homolog, sarcoma antigen NY-SAR-95, spermatogenesis associated 30
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INTS13 using anti-INTS13 antibody (A32419-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS13 antigen affinity purified polyclonal antibody (Catalog # A32419-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INTS13 at approximately 85 kDa. The expected band size for INTS13 is at 80 kDa.
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