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ATF1 Antibody (YA3136, Rabbit)

HY-P83391
MedChem Express
TargetATF1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ATF1 Antibody (YA3136, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID466
  • Target name
    ATF1
  • Target description
    activating transcription factor 1
  • Target synonyms
    EWS-ATF1, FUS/ATF-1, TREB36, cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ATF1 Antibody (YA3136) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3136), targeting ATF1, with a predicted molecular weight of 29 kDa (observed band size: 36 kDa). ATF1 Antibody (YA3136) can be used for WB, ICC/IF, IP experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ATF1 using anti-ATF1 antibody (PB9130). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF1 antigen affinity purified polyclonal antibody (Catalog # PB9130) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF1 at approximately 38 kDa. The expected band size for ATF1 is at 38 kDa.
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