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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA012203ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA012203ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA012203ESR1HU at dilution of 1:100

ATG13 Antibody

CSB-PA012203ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATG13
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Overview

  • Supplier
    Cusabio
  • Product Name
    ATG13 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9776
  • Target name
    ATG13
  • Target description
    autophagy related 13
  • Target synonyms
    ATG13 autophagy related 13 homolog; autophagy-related protein 13; KIAA0652; PARATARG8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75143
  • Protein Name
    Autophagy-related protein 13
  • Scientific Description
    Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIAA0652 using anti-KIAA0652 antibody (PB9480). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA0652 antigen affinity purified polyclonal antibody (Catalog # PB9480) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA0652 at approximately 56 kDa. The expected band size for KIAA0652 is at 56 kDa.
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